PCRTiler: automated design of tiled and specific PCR primer pairs
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چکیده
منابع مشابه
PCRTiler: automated design of tiled and specific PCR primer pairs
Efficiency and specificity of PCR amplification is dependent on several parameters, such as amplicon length, as well as hybridization specificity and melting temperature of primer oligonucleotides. Primer design is thus of critical importance for the success of PCR experiments, but can be a time-consuming and repetitive task, for example when large genomic regions are to be scanned for the pres...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2010
ISSN: 1362-4962,0305-1048
DOI: 10.1093/nar/gkq485